Expression of rat Ian5 in tissues from lyp/lyp, lyp/+, and +/+ DR BB rats. (a) Northern blot containing 3 μg of poly(A⁺) RNA from thymus, spleen, or kidney of each of +/+ or lyp/lyp rats probed with a 695-bp region of Ian5showing a 1.4-kb transcript (Ian5). The blot was stripped and reprobed with a 1420-bp GAPDH probe (GAPDH). The images were quantitated using a PhosphorImager and software and normalized to GAPDH expression in each lane. Size markers are indicated. (B) Northern blot containing 3 μg of poly(A⁺) RNA from thymus, spleen, lymph node, and kidney from each of +/+, +/lyp, andlyp/lyp rats probed as in A. Size markers are indicated. (C) Methylene blue stain of the blot in Bbefore probing, showing even loading of 18S ribosomal RNA in each lane.