Example of potential annotation errors. Comparison of GenBank annotation (CDS field) and SGP-1 prediction for human and mouse insulin genes (accession nos. M10039 and X04724). Fromleft to right, the fields are identifier (1), source (2), feature (3), sequence positions of beginning and end of a coding exon (4 and 5), score (6), strand (7), reading frame (8), grouping (9), and sequence (10). Discrepancies between annotation and prediction are marked by black circles around murine donor and acceptor positions and reading frame for exon 2. Capital letters indicate the coding sequence.