Assessment of distinct SNPs on single fragments using real-time pyrophosphate detection. Arrowhead doublets indicate the location of SNPs. The schematic illustration below each pyrogram series shows the template, the primer location (arrow), and incorporated nucleotides (bold letters). (a) Variations in the human angiotensinogen promoter. Genotypes shown (at bottom) are: G/G, A/A and G/A (G-6A) and A/A, C/C and A/C (A-20C). (b) Polymorphisms, located in the promoter of human angiotensin II type I receptor gene. SNP configurations shown (top to bottom) correspond to G/G, C/C and G/C in position C-226G and A/A, C/C and A/C, in position A-227C.