Representative Northern blots of new transcripts. New transcripts were analyzed for message size and expression levels by probing one or more of the three commercially available multiple tissue Northern blots. (A, left) Endocrine blot probed with a 1.8-kb Mu cDNA from the Mu transcript; (middle) blot probed with a 220-bp Mu cDNA. In both cases a 2.8-kb message is identified that is expressed in all tissues. (A, right) Blot containing total RNA from EBV-transformed lymphocytes from seven FMEN1 cases, probed with the 8.8-kb Epsilon/Beta probe. Two messages are seen: a more abundant 4.4-kb Epsilon transcript and a less abundant 19-kb message named Beta. (B) MucDNA and location of probes used for blots in A left andmiddle. (C) Epsilon/Beta cDNA and probes used in A right. Probes used in A are in Band C. The 2.8-kb probe in C independently confirmed the 19-kb Beta transcript, and did not detect Epsilon(data not shown).