The graphic view of the multiple alignments for the MEN1 gene against mouse and human EST database. Annotated mRNA and coding features, shown as shaded and open boxes respectively, are labeled above the alignments. Each box represents an exon, and the mRNA sequence is made up of 10 exons. Exons 1,2,3,7,8,9, and 10 are confirmed by the mouse EST hits. Exons 2,5,6,7,8, and 10 are confirmed by the human EST hits. Combining the results from both the mouse and human database, only exon 4 is missing from the EST hits. Two human ESTs hits (GenBank accession nos. AA209475 and AA211877) are aligned to the intronic regions. They are the 5′ and 3′ ends of the same cDNA clone (648332) sequenced by the Washington University School of Medicine (St. Louis, MO). The alignments are in reverse orientation of the transcription, which suggests a potential antisense transcription of this gene.