YMR074C is an inhibitor of the Slt2-MAP kinase cell wall integrity pathway. (A) Overrepresented triplet motif that identified YMR074C to be SDL with two kinases of the cell wall integrity pathway, Bck1, and its downstream target Slt2. (B) Model describing the role of Ymr074c as an inhibitor of cell wall integrity. (C) Overnight cultures of bck1Δ, slt2Δ, ymr074cΔ, single or double mutants were serially diluted and spotted onto YPD medium in the absence (left) and presence (right) of 10 mM caffeine. (*) Phenotypic rescue.