ncRNAs associated with Dlx1/Dlx2 and Dlx5/Dlx6 loci. (A,B) Genomic context of the Dlx1/Dlx2 (A) and Dlx5/Dlx6 (B) loci showing the position of the Dlx genes (blue), ncRNAs (Dlx1as and Evf1, light red; Evf2, dark red), and the highly conserved enhancers (I12a [Park et al. 2004]; VISTA ID290 [Pennacchio et al. 2006]; m1561, [Zerucha et al. 2000]; green). Arrows indicate the direction of transcription. (C,D) Relative expression profiles of Dlx1 and Dlx1as (C) and Evf1, Evf2, and Dlx6 (D) during EB differentiation as determined by qRT-PCR (relative to day 0 or 1; primer positions indicated in A,B). Error bars show standard deviation (SD) determined from at least three replicates. (E–G) ISH of sagittal adult mouse brain sections for Dlx1 (E), Dlx1as (F), and Dlx2 (G). Whole brain is shown in the left panels; subventricular zone (SVZ), rostral migratory stream (RMS), and olfactory bulb (OB) in the middle panels; and the hippocampus (HP) in the right panels. Dlx1, Dlx1as, and Dlx2 show similar expression in the OB, RMS, and SVZ in the brain and in addition Dlx1as is strongly expressed in cells dispersed throughout the cortex (CX) and HP.