Comparison of the sensitivity and specificity of CRASA with the other 13 annotation tools. (A) The sensitivity (S_n , closed circle) and specificity (S_p , open circle) are computed from the annotation results of the RG data set by the indicated homology- and ab initio-based tools. (B) The exon-level accuracy in terms of missing exon (ME, closed circle) and wrong exon (WE, open circle) is calculated from the same results described inA. The mean values of S_n ,S_p, ME, and WE of these 14 tools are, respectively, 0.54, 0.47, 0.29, and 0.38, whereas those of CRASA are 0.92, 0.79, 0.05, and 0.1.