Variation in t _4D, t _AR,t _intron, t _coding,t _3′UTR, and t _5′UTR along human Chromosome 22 (A) and residuals oft _4D, t _AR,t _intron, t _coding,t _3′UTR, and t _5′UTR after quadratic regressions on human CG content along human Chromosome 22 (B). All values were calculated from 5-Mb overlapping by 4-Mb windows of the human–mouse alignment and were normalized using the genome-wide mean and standard deviation (denoted by superscript + inA and * in B). The normalization was done to ensure that all values have the same dynamic range.