S. cerevisiae cell microarrays for assaying the drug target of rapamycin. Ninety four yeast homozygous deletion strains, includingfkb1 strain and two negative controls, were arrayed with five repeats in a total of 576 nanocraters. fkb1 strain was arrayed at 8A to 8F; two negative controls that did not contain any cells were arrayed at 15M to15R and 18S-18X. One set of cell microarrays was grown on YPD and the other on YPD containing 1 μg/mL of rapamycin at 30°C overnight.