The CRAWDAD algorithm was used to align traces. Raw data collected using the 32Karat software show variability in migration time depending on the date of sample collection. The software program CRAWDAD uses dynamic time warping to achieve optimal alignment of raw data by minimizing distance between traces. Five traces from five different plates are plotted before (left) and after (right) alignment with the two steps of the CRAWDAD alignment. Time is plotted along the x-axis in minutes; intensity in relative fluorescent units is plotted along the y-axis.