Cellular pathways regulated by differentially expressed miRNAs in cancer. KEGG analysis by ClueGO (Bindea et al. 2009) of pathways (score = 3, P-value < 1 × 10−3) simultaneously targeted by both up-regulated and down-regulated miRNAs (listed in Table 1; Supplemental Tables IV, V). The KEGG pie-chart shows the functional effect of differentially expressed miRNAs on cellular pathways in cancer. The large majority of the affected pathways is related to cancer or signal transduction (i.e., Wnt, VEGF, TGF-beta, insulin, and phosphatidylinositol signaling, focal adhesion, and colorectal cancer). Target genes selection was performed with DIANA-miRpath, microT-V4.0 (Papadopoulos et al. 2009). The union of the target mRNAs with a score above 3 was used as an input to ClueGO. Right-sided hypergeometric test yielded the enrichment for GO terms. Benjamini-Hochberg correction for multiple testing controlled the P-values. GO term fusion was applied for redundancy reduction.
