(A) Distribution of surface electrostatic potential on the DNA binding interface of Myb/SANT domains. The top row shows DNA binding Myb domains that have a basic surface charge (blue) on their DNA binding interface, and the bottom row shows SANT domains known to be involved in protein-protein interactions that have an acidic (red) or mixed charged surface in the same region. (B) Multiple sequence alignment of a representative set of DNA binding Myb domains (top) and SANT domains (bottom). Proteins are denoted by their gene names or pdb id (where a structure is available), their species abbreviations, and the Genbank Identifier (gi). The coloring reflects the amino-acid conservation profile at 90% consensus. The + charge in the minor groove binding site is shown to the right. Species abbreviations are as follows: At, Arabidopsis thaliana; Bs,Bacillus subtilis; Dm, Drosophila melanogaster; Gaga,Gallus gallus; Hs, Homo sapiens; Mm, Mus musculus; Sc, Saccharomyces cerevisiae; Zm, Zea mays.