Electropherogram of a pUC-18 subclone with a random 3- to 4-kb human DNA insert purified with this protocol. Plasmid was sequenced with ET dye-terminator chemistry from Amersham Pharmacia Biotech on a Molecular Dynamics MegaBACE 1000 with standard conditions. Read length was 805 bp, scoring a Phred Q20 or greater.