Analysis of BAC clones containing α-satellite sequence. Thirty-seven α-satellite positive BAC clones were isolated and digested with NotI or EcoRI. (A) TheNotI-digested DNA was separated using CHEF with the Low Range PFG Marker loaded on both sides, and the sizes of the markers are indicated with arrows. (B) The EcoRI-digested DNA was fractionated with standard agarose gel electrophoresis with the 1-kb DNA ladder (outside) and the λ DNA/HindIII fragments (inside) loaded on both sides. Vector bands that are indicated by arrows are observed at 8.8 kb in both panels. False-positive clones that contain intact vector are shown in lanes 5 and39 in both panels. Stronger intensity of the vector bands is due to the high copy number of plasmid derived from pUC19-stuffer fragment. A variety of EcoRI repetitive blocks are observed inB and indicated with vertical arrows.