Physical map of the ZNF gene cluster in 19p12. The organization of the region under study is depicted at three levels of resolution. (a) An ideogram of chromosome 19 delineates the ∼1.5-Mb portion from the 4- to 5-Mb region of the ZNF cluster that has been characterized. (b) Cytogenetic distances between representative cosmids are shown based on estimates of physical distance from three-color FISH analysis on decondensed chromatin from sperm pronuclei. Cosmids used in the construction of the framework cytogenetic map are indicated below each contig (shown as open horizontal bars) in association with genetic markers for the chromosome 19 map. (c) Overlapping BAC and cosmid genomic clones for 700 kb of this region are shown as horizontal lines. The orientation and order of clones in the contig were determined based on anEcoRI fingerprinting strategy of multiple overlapping clones. Only a subset of the clones in the total tiling path of these clones is indicated. The positions of cross-hybridizing probes specific for the KRABA exon (A), spacer region (S) and zinc finger gene (Z) ofZNF91 are indicated within the physical map. Vertical shaded bars indicate the position and extent of regions that cross-hybridize to β-satellite probes derived from BAC 33152. The two clones that have been sequenced are indicated with an asterisk (*).