Ty elements transpose into widely dispersed chromosomal targets. The upstream flank of RNA polymerase III-transcribed genes including tRNA (red dots), 5S, U6, and P1 RNA (gray dots) genes are targets for Ty1, Ty2, Ty3, and Ty4 transposition. Ty5 transposes to silenced loci including telomeres and mating-type cassettes on chromosome III (green dots).