Experimental strategy for performing DNase-seq. G1E and G1E + GATA1 cells were grown asynchronously or arrested in mitosis by nocodazole treatment. Mitotic populations of nocodazole-treated cells were isolated by FACS using intracellular antibody staining of H3S10Ph. All samples were subjected to DNase digestion, followed by affinity capture of cleaved fragments, library preparation, and sequencing.