Single-molecule sequencing protocols. The standard single-molecule sequencing protocol using tailed DNA and oligo(dT) flow cells (left) and the novel protocol using Direct Capture (right). The diagram on the left shows three steps in the standard protocol, while the diagram on the right shows the hybridization step only for the Direct Capture protocol with three different possible types of hybridizing molecules: a DNA that is only partially complementary to the capture primer (underhang), a DNA that is perfectly complementary to the capture primer, and a DNA that has a 3′ overhang. All of these molecules are sequenceable.