eQTLs in biological replicates of primary human osteoblasts. All expression traits from two biological replicates (replicate 1 = R1 and replicate 2 = R2) of 94 cultured human osteoblasts were tested in separate analysis for association with SNPs with putative cis- or trans-regulatory effects. The overlap between the replicates (white bars) was calculated at different P-value thresholds of R1 ranging from P < 5 × 10−8 – 1 × 10−4 (x-axis) and compared with the average statistical power (black bars) to detect the differences using alpha = 5 × 10−4 (R2) and effect sizes (r2) corresponding to each significant eQTL in R1. (A) cis-eQTLs are defined as expression traits associated with SNPs located ±250 kb region flanking either side of the gene. (B) Trans-eQTLs include SNP–gene associations located on different chromosomes.
