Chromosomal localization of the marsupial, cat and lemur orthologs as determined by fluorescence in situ hybridization analysis. (A) and (B) Localization of the HSPRY3 and CXYorf1 genes on chromosome 3. (C) The position is confirmed after sequential hybridization with HSPRY3 and tammar chromosome 3 paint. (D) and (E) IL9R and SYBL1 hybridized, respectively, to chromosome 1 and X. (F) Simultaneous hybridization ofCXYorf1 and HSPRY3 to chromosome 3 whereCXYorf1 is yellow (G) and HSPRY3 signal is red (H). (I) and (J) Chromosomal localization of the cat and lemur orthologs as determined by fluorescence in situ hybridization analysis. (I) Localization of IL9R to chromosome E3 in the cat, and (J) HSPRY3 to the X chromosome in the Lemur.