RT-PCR for several genes. (A) CG10174, (B)CG13732, (C) CG17856, (D)CanB, and (E) CG9873. Lane 1corresponds to gonadectomized male cDNA, lane 2 is testis + accessory glands cDNA; lanes 3 and 4 are the negative controls after DNA digestion for the experiments of lanes1 and 2, respectively, and lane 5 is the negative control of the PCR. Lane 6 is the PCR experiment using testis cDNA; lane 7 is the negative control after DNA digestion, and lane 8 is the negative control of the PCR. (F) Lane 1 is CG15645 RT-PCR using cDNA from polyA selected RNA from a mixed sample of males and females; lane2 is the PCR from this mRNA without being reverse-transcribed from the mixed sample; lanes 3 and 4 are the nested PCR experiments using the PCR products of lanes 1 and2 as templates. The DNA marker, as shown here, is a 1-kb DNA ladder (Gibco).