Cumulative curve of unique tag detection through sequencing of 152,791 tags from normal male and Ts65Dn male and normal female mice brain serial analysis of gene expression (SAGE) libraries. A total of 22,115, 21,357, and 19,103 unique tags were detected after sequencing 51,561, 50,504, and 50,726 tags from normal male, Ts65Dn male, and normal female mice brain SAGE libraries, respectively.