Drosophila developmental life cycle proteome. (A) Scheme depicting the collected time points throughout the four major metamorphic stages of Drosophila (embryo [red], larva [blue], pupa [green], and adult [violet]). (WP) White pupa, (L3c) crawling third instar larva. (B) Heat map of log₂ LFQ values of the 7952 protein groups quantified during fly development. (C) Visualization of the first two principal components separating samples according to their developmental stage. The biological replicates are indicated in the same color, with elliptic areas representing the standard error of the two depicted components.