Scalability of solid-phase reverse transfection of proteins. (A) A schematic representation of a 384-well plate coated with GFP containing transfection complexes using condition I (Supplemental Table S1) (gray colored wells): (X) wells that were imaged. (B) The robustness of solid-phase reverse transfection of GFP. HeLa cells were seeded on the precoated wells, incubated for 24 h, fixed, and imaged. Nuclei were stained with Hoechst 33342. (Scale bar) 20 µm.