Molecular diversity underlying classical visible phenotypes. (A) Two strains exhibiting the yellow body color phenotype carry the previously characterized SNP that disrupts the start codon. In contrast, strain 6027 harbors a 622 bp insertion in the second exon, resulting in a frameshift and a premature stop codon. (B) Phenotype images of wild-type (top) and y¹ mutant (bottom) body color. (C) The forked (f¹) allele was previously associated with a TE insertion; however, strain 1570 lacks this insertion and instead carries a 2.7 kb tandem duplication encompassing a complete exon. (D) Phenotype images of wild-type (top) and f¹ mutant forked bristles (bottom, blue arrow). (E) Two alleles of the white gene are associated with TE insertions near the transcription start site. A 4.7 kb Doc element insertion is present in strains 1570 and 6027, and a nested copia element within the Doc insertion is observed in strain 5295, consistent with recurrent insertions at the same genomic site. (F) Phenotype images of wild-type (top) and white mutant (bottom) eye color. (G) Two alleles of garnet involve either an intronic blood element insertion (g¹) or a nonsynonymous SNP with an in-frame deletion in the final coding exon (g²). (H) Phenotype images of wild-type (top) and g¹ mutant eye color, obtained from FlyBase (FBrf0220532).
