Figure 4.

Molecular diversity underlying classical visible phenotypes. (A) Two strains exhibiting the yellow body color phenotype carry the previously characterized SNP that disrupts the start codon. In contrast, strain 6027 harbors a 622 bp insertion in the second exon, resulting in a frameshift and a premature stop codon. (B) Phenotype images of wild-type (top) and y¹ mutant (bottom) body color. (C) The forked (f¹) allele was previously associated with a TE insertion; however, strain 1570 lacks this insertion and instead carries a 2.7 kb tandem duplication encompassing a complete exon. (D) Phenotype images of wild-type (top) and f¹ mutant forked bristles (bottom, blue arrow). (E) Two alleles of the white gene are associated with TE insertions near the transcription start site. A 4.7 kb Doc element insertion is present in strains 1570 and 6027, and a nested copia element within the Doc insertion is observed in strain 5295, consistent with recurrent insertions at the same genomic site. (F) Phenotype images of wild-type (top) and white mutant (bottom) eye color. (G) Two alleles of garnet involve either an intronic blood element insertion (g¹) or a nonsynonymous SNP with an in-frame deletion in the final coding exon (g²). (H) Phenotype images of wild-type (top) and g¹ mutant eye color, obtained from FlyBase (FBrf0220532).

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