Figure 1.

Extracted RNA from fresh, silica-dried, and herbarium specimens provide usable transcriptomes based on bioinformatic metrics. (A) The RNA integrity (RIN) scores for samples used in this study. (B) The proportion of contigs inferred to be “good contigs” compared with the proportion inferred as “bad contigs” based upon the TransRate metrics. (C) The inferred completeness of the transcriptomes based upon sequenced single-copy orthologs. (D) The total number of inferred orthologs from each sample used to infer the phylogeny. (E) The coalescent-based inferred phylogenetic relationships and sample source for transcriptomes in this study. Branch lengths converted to molecular rates (subs/bp).

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