Molecular cytogenetic validation of chromosomal rearrangements across batches and subcultures
| BATCH1_PREP1A | BATCH2_PREP2A | BATCH2_PREP2B | BATCH2_PREP2C | BATCH2.1_PREP2.1A | BATCH2.1_PREP2.1B | BATCH2.1_PREP2.1C | BATCH3_PREP3A | |
|---|---|---|---|---|---|---|---|---|
| Exp1 t(1;22) | 1b/22b n = 5 | 1b/22b n = 3 | 1b/22b n = 20 | 1b/22b n = 20 | 1b/22b n = 2 | Not tested | Not tested | 1b/22b n = 3 |
| Exp2 inv(7) | 7b n = 4 | 7b n = 3 | 7b n = 22 | 7b n = 20 | 7b n = 2 | Not tested | Not tested | 7b n = 5 |
| Exp 3 inv(11) | Heta | Het (Dir = 8; Inv = 8) | Het (Dir = 12; Inv = 8) | Het (Dir = 7; Inv = 10) | Het (Dir = 11; Inv = 14) | Not tested | Not tested | Het (Dir = 6; Inv = 4) |
| Exp 4 inv(7) | Heta | Not tested | Het (Dir = 10; Inv = 9) | Het (Dir = 7; Inv = 10) | Het (Dir = 9; Inv = 8) | Not tested | Not tested | Not tested |
| Exp 5 inv(22) | Het (Dir = 16; Inv = 16) | Het (Dir = 17; Inv = 2) | Het (Dir = 5; Inv = 4) | Het (Dir = 3; Inv = 2) | Het (Dir = 33; Inv = 4) | Het (Dir = 39; Inv = 7) | Not tested | Het (Dir = 7; Inv = 7) |
| Exp 6 iso(14) | T14 n = 2 | i(14p) = 77,5% i(14q) = 22,5% n = 49 | i(14p) = 33,3% i(14q) = 66,7% n = 18 | i(14p) = 50% i(14q) = 50% n = 2 | i(14p) = 90,9% i(14q) = 9,1% n = 11 | Not tested | Not tested | T14 n = 5 |
| Exp 7 iso(14) + inv (22) | Not tested | Not tested | Iso-p + dir/dir = 36,8% Iso-q + dir/inv = 63,2% n = 19 | Iso-p + dir/dir = 28% Iso-q + dir/inv = 72% n = 25 | Iso-p + dir/dir = 65% Iso-q + dir/inv = 35% n = 20 | Iso-p + dir/dir = 56,3% Iso-q + dir/inv = 43,7% n = 16 | Iso-p + dir/dir = 87% Iso-q + dir/inv = 13% n = 23 | Not tested |
[i] Results of FISH experiments validating the presence and configuration of major chromosomal rearrangements in multiple metaphase preparations from the Nomascus siki cell line. (n) Number of observed metapahses or interphase nuclei, (dir) number of chromosomes showing direct orientation, (inv) number of chromosomes showing inverted orientation, (Het) heterozygous, (Iso) isochromosome, and (T) trisomy.
[ii] aMercuri et al. (2022).