Overview of the ChromMovie algorithm. (A) The algorithm takes an ordered list of scHi-C maps representing different time points of a cellular process. It then simulates all time points simultaneously, generating an ordered list of 3D structures as the result. (B) The four main types of forces used in the ChromMovie simulation: repulsive forces, backbone forces, contact forces, and frame forces. (C–E) Three approaches to modeling multiple ordered scHi-C maps: independent modeling (C), sequential modeling (D), and parallel simultaneous modeling (E). ChromMovie implements a form of simultaneous modeling, which, to our knowledge, is the first of its kind in the field of chromatin modeling.