ACTB H73 methylation is required for the colocalization with SMARCA4. (A) Whole-cell lysates of HCT-116 Ct, SETD3 KO#1, SETD3 KO#2; cell lines were separated on a 10%–30% glycerol gradient. The indicated fractions were then subjected to western blot assay using the indicated antibodies. (B) Same as A with HCT116 cells expressing Flag-ACTB wt (WT ACTB) or Flag-ACTB H73A (MUT ACTB H73A).