Restoration of euploidy using the KaryoCreate system. (A) Schematic describing the use of the KaryoCreate system to induce the missegregation of a specific chromosome. (B) Copy numbers of CSMD1, MICU3, and CRISPLD1 (a gene located on the q arm of Chromosome 8), in trisomic proband clones that were transiently transfected with KaryoCreate plasmids. Mean ± SEM; data from representative trials are shown (n ≥ 3 total trials). Genomic DNA extracted from MCF10A cells was used as a diploid control. (C) Karyotype of the euploid revertant clone 31.3.