PRINS signals using the left and right ITRs of Hsmar2. (A) Raw PRINS data. This is an unenhanced chromosomal spread of the biotin-labeled left ITR. The green signals represent locations of Hsmar2 elements that contain left ITRs that match the consensus for Hsmar2 with enough homology to promote annealing and priming for the PRINS signal. (B) An example of the analysis of PRINS signals on chromosome 17. Twelve independent PRINS experiments are represented here by the 12 individual chromosome 17 images. The green signals are PRINS labeling using the left ITR. Note that not all chromosomes show all of the signals and that some chromosomes only show a signal for one homolog. This limitation of the technique requires the analysis of multiple chromosomal spreads. The signals indicated by numbers 1–4 are located at 17p12, 17q12, 17q22, and 17q24-25, respectively, at a resolution of 400 chromosomal Giemsa bands. (C) Summary of all left and right ITR PRINS data. This is a karyotype representing all loci that contain Hsmar2 elements by PRINS. The image was constructed by combining the PRINS signals for each individual chromosome into a composite image that was then added to other chromosomal images and arranged into the karyotype.