In vitro reconstruction of chromosome 7. After the elimination of 24 probes and 264 hits, hybridization and FPC, and genetic data were in excellent agreement. Eight anchored contigs were merged by chromosome walking into five contigs (purple bars), which covers ∼4.1 Mb of the 4.2-Mb chromosome. The genetic map, shown as the blue bar, is contiguous between the top right and bottom left. The comparison of genetic and physical distance revealed an average value of 41 kb/cM. Ratios varied from 19 to 180 kb/cM. After removal of redundant BAC clones from all the contigs, a minimum tiling path was created as indicated by short overlapping colored lines. The name of each clone is indicated beneath. The interval between each BAC clone is ∼100 kb with ∼30 kb of overlap. The color scale indicates the depth of BAC clone coverage for each region. The coverage ranges from 1 to 24 clones per 100 kb with an average of 7 clones.