Description of Minisatellite Probes Identified from PACs and Cosmid Clones
|
PACs
(RPCI6 no.) | Localization | Minisatellite(s) within | Fragment | Heterozygote frequency | No. of alleles | AluI allele size (kb) |
| 19 (213 J16) | 2p23 (F) | UPS19 | AluI–HinfI (1.7) | 0/16 | 1 | (1.8; 1.4; 1.3) allele cut |
| 34 (196 L17) | 4p16 (L) | UPS14 | AluI–HaeIII (2.8) | 7/16 | 3 | 4; 2.7; 2.4 |
| (ter) | ||||||
| 28 (202 F12) | 5p15.3 (L) | UPS9 | AluI–HaeIII (2.1) | 10/16 | 3 | 2.6; 2.4; 1.9 |
| (ter) | ||||||
| 32 (208 C19) | 6p21 (F, L) | UPS8 | HaeIII–HinfI (2.1) | 4/4 | 6 | 3.3; 2.4; 1.6; 1.65; 1.55; 1.3 |
| 21 (195 E8) | 7p22 (F, L) | UPS5 | HaeIII–HinfI (1.6) | 3/4 | 3 | 1.9; 1.75; <0.5 |
| (ter) | ||||||
| 33 (238 P2) | 11q25 (L) | UPS3 | AluI–HaeIII (1.7) | 10/16 | 4 | 1.95; 1.90; 1.85; 1.7 |
| (ter) | ||||||
| 6 (223 C10) | 12p13.3 (F) | UPS15 | AluI–HaeIII (1.5) | 0/16 | 1 | 2.2 |
| (ter) | ||||||
| 26 (207 H21) | 12q24.33 (F) | UPS20 | AluI–HinfI (1.7) | 0/16 | 1 | 1.9 |
| (ter) | ||||||
| 1 (217 O7) | 13q34 (F, L) | UPS17 | AluI–HinfI (2.4) | 8/16 | 4 | 2.9; 2.85; 2.4; 1.8 |
| (ter) | ||||||
| UPS21 | AluI–HinfI (1.3) | 7/16 | 2 | 1.8; 1.3 | ||
| UPS22 | AluI–HinfI (1.8) | 5/6 | 2 | 1.8; 1.6 | ||
| 50 (210 M23) | 13q34 (F, L) | UPS6 | AluI–HinfI (1.6) | 8/16 | 3 | 1.8; 1.6; 1.3 |
| (ter) | ||||||
| UPS7 | AluI–HaeIII (1.9) | 13/16 | 6 | 2.25; 2.20; 2.15; 1.95; 1.75; 1.65 | ||
| 13 (224 C15) | 17q25 (F, L) | UPS12 | AluI–HaeIII (1.5) | 2/16 | 3 | 1.65; 1.6; 1.55 |
| (ter) | ||||||
| 25 (213 P13) | 17q25 (L) | UPS4 | HaeIII–HinfI (1.45)/ | 10/16 | 4 | 1.95; 1.7; 1.65; 1.55 |
| (ter) | AluI–HaeIII (1.6) | |||||
| 36 (238 N11) | 21p13 (L) | UPS13 | HaeIII–HinfI (2.9)/ | 3/4 | 7 | 4.3; 3.8; 3.0; 2.6; |
| (ter) | HaeIII–HinfI (3.1) | 2.55; 2.4; <0.5 | ||||
| 49 (240 M9) | 18q23 (L) | UPS1 | AluI–HaeIII (2.4)/ | 15/16 | 7 | 4.2; 3.8; 3.75; 3.5; |
| (ter) | HaeIII–HinfI (2.5) | 3.45; 3.0; 2.9 | ||||
| 24 (231 K15) | 22q13 (F) | UPS11 | AluI–HinfI (1.4) | 6/16 | 2 | 1.8; 1.6 |
| (ter) | ||||||
| Cosmids | Name | HinfI or HaeIII allele size (kb) | ||||
| c112–J06107 | 1p36.6 (F, L) | CEB121 | HaeIII (0.6) | 12/16 | 5 | 1; 1.4; 1.45; 1.75; 1.8 |
| (ter) | (HinfI) | |||||
| c112–J2362 | 5p15.3 (F, L) | CEB119 | HaeIII (1.5) | 11/16 | 7 | 1; 1.2; 1.35; 1.4; 1.55; |
| (ter) | 1.8; 4.2 (HinfI) | |||||
| c112–I0724 | 8q24.3 (F, L) | CEB118 | HaeIII (1.2) | 4/16 | 2 | 1.05 + 1; 0.95 + 1.8 |
| (ter) | (HaeIII) | |||||
| c112–M1148 | 10q26 (F, L) | CEB117 | HaeIII (0.5) | 9/16 | 4 | 5.4; 5.5; 5.6; 6.5 |
| (ter) | (HinfI) |
[i] In three cases, UPS15, UPS19, and UPS20, only one allele was detected. We assume that these sequences are tandem repeats because of the strong signal intensity obtained on Southern blots and because of the large allele size detected on AluI, HaeIII, andHinfI digests (frequent cutters). (ter) Terminal band.