Table 2.

Effect of Eliminating Replicate Ditags on Tag Counts

Tag (CATG + . . .) Identity Frequency including replicate ditags Frequency excluding replicate ditags Decrease after excluding replicates (%)
CCCATCGTCCCOX2134889034
AAGATCAAGAactin75652331
TGATTTCACTCOX331624024
GGGGAGGAACslow troponin T20516918
CAAGTATAAAtitin13411713
TTTACTCAGCC protein58555
CCTCTCTGGTEST F20783 34340
TCTTGTGCATLDH A10100
AACAGCTCCCEST F19683 660

[i] These tag counts do not match those in Table 1 because they represent only half of the data. Because the underestimation of the most abundant tags would have become progressively greater with an increasing number of total tags, two separate databases were created and merged to produce the data in Table 1. Thus, the underestimation for the combined databases does not exceed the percentages shown here. (COX2) Cytochrome c oxidase 2; (COX3) cytochrome c oxidase 3; (LDH A) lactate dehydrogenase A.