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Table 1.

Cloning and Expression Results for Yeast Genome and Human Kinase Studies

Yeast first-pass process		No. successful		No. attempted		Success rate (%)
Yeast first-pass process		pYES2	pcDNA3.1	pYES2	pcDNA3.1	pYES2	pcDNA3.1
ORF amplification	5632		6035		93	93
Topoisomerase I-mediated cloning + orientation	3749	3762	5632	5632	67	67
Expression QC positive	659	558	3749	3762	18	15
Start-to-finish summary	659	558	6035	6035	11	9
Yeast second-pass process
ORF amplification	5000	5093	5376	5477	93	93
Topoisomerase I-mediated cloning + orientation	3750	3266	5000	5093	75	64
Expression QC positive	1553	1197	3750	3266	41	37
Start-to-finish summary	1553	1197	5376	5477	29	22
Human kinase process	No. successful (pcDNA3.1)		No. attempted (pcDNA3.1)		Success rate (%) (pcDNA3.1)
RT–PCR	179		288		62
Topoisomerase I-mediated cloning + orientation	140		179		78
Expression QC positive	108		140		77
Start-to-finish summary	108		288		38

[i] Cloning and expression results for the yeast genome and human kinase studies. Success rates are expressed as percentages of successful attempts over the total number of attempts for each step of the cloning and expression process.