Sequencing the Mouse KCC2 Locus with the Mu System
| Plasmid | pSR11 |
| Insert size (kb) | 10.3 |
| Vector size (kb) | 2.9 |
| Minitransposon used | cat–Mu |
| E. coli cells used | |
| Strain | DH5α |
| Competence (cfu/μg pUC19 DNA) | ∼107 |
| Plasmid introduced into cells by | electroporation |
| Fraction of standard reaction used in electroporation | 1:10 |
| Number of ApR/CmRcolonies obtained | 540 |
| Number of clones screened by restriction analysis | 95 (100%) |
| two plasmids apparent | 2[ii] (2%) |
| one plasmid apparent | 93 (98%) |
| Clones with one transposon | 93 (98%) |
| in vector | 14 (15%) |
| in insert | 79 (83%) |
| Number of clones sequenced | 71 (75%) |
| Total sequenced (bp) | 68,143 |
| Final sequence (bp) | 10,288 |
| Average read per sequence reaction | |
| (nucleotide) | 494 |
| Redundancy (fold) | 6.6 |
[i] Sequences retrieved using RP and UP primers are included.
[ii] These clones exhibited a restriction pattern consistent with cotransformation of two plasmids.