Oligonucleotides Used in this Study
| Locus | First-round primers (5′ to 3′) | Second-round primers (5′ to 3′) |
| Feline | ||
| AMELX | F-CTACCACCTCATCCTGGGCA | F-TTCCCATTACCAGCTTCCTG |
| R-TTGGTGGTGCAGCCATCCAC | R-CCCCAACTTTTACAGGCTGA | |
| ARAF1 | F-GGACCTCAGCAAAATCTCCA | R-GCCTAATGGGGAAGTACATC[i] |
| R-GCTCAATCTTGGGGAGTGAC | ||
| ATP7A(mnk)[ii] | Venta et al. (1996) | F-CTCTCAAGGTCTCTGGAGAA[i] |
| BGN | Lyons et al. (1997) | F-CAGGAAAGGCTCAGGGAGAG[i] |
| BTK | Lyons et al. (1997) | F-AACACAAATCCTGTCTCCATCA |
| R-TTCAGTCAGCAGTGGAGCTG | ||
| DBX | F-TATTTTGGGGTAGTGTGAGC | F-CAGATTCTCAGATGTTTGTTGC |
| R-GGCCACGCGTCGACTAGTAC[iii] | R-ACATGTTTGAGGATAGCAATGT | |
| DFFRX | F-GACTGCAGTAACTCAGAGGA | F-CTGTTAATTCTTAAGCAAGAAAC |
| R-GGCCACGCGTCGACTAGTAC[iii] | R-TTGCAGAGTGCAATGGGTAG | |
| DMD | F-AAGTGGAAATGTTGCCAAGG | R-CACACACACACACACGCATT[i] |
| R-AGGTGGTGACATAAGCAGCC | ||
| F9 | Lyons et al. (1997) | |
| IDS | F-ACATTGACCAACTGGCATCC | |
| R-CACCGACATGGTCACATAGC | ||
| MAOA | F-GGCACTACTCGGATATTCTC | |
| R-ACTTGACCAGATCCACCTAC | ||
| PHKA2 | F-GTGGGAGCGTGGAGATAAGA | F-GTGGCAACATCCAGGAAAGT |
| R-TGAATCACTGACTTGCGTCC | R-ACAAGCACAGGTGTGAATCAG | |
| PLP | F-GGCCACTGGATTGTGTTTCT | |
| R-TAGTCGCCAAAGATCTGCCT | ||
| RPS4X | F-TGATGGCAAGGTCCGAACTG | |
| R-TGGTGTCATAGATCAGGCGG | ||
| SMCX | F-TCAGGGGCTGAAGAAGAAGA | F-CCCCCACACTAGATGCAGAT |
| R-TGGACCCACAGTGACTGGTA | R-TCTCTTCCCAGACCTCTCCA | |
| UBE1X | Murphy et al. (1999) | F-GCACGGGGATCTCAGAAGA |
| R-AATGCCACAGGTAAAGGAGA | ||
| UTX | F-CACCCTCTTCAGCCATTTC | F-GACGAATCCTAGCAACCAGC |
| R-GTTGGTGGTCTTGGAGGTG | R-CCTGAGTGTCATGGGGAAAC | |
| XIST | F-TGTTCCAGGCCAATGAGAAG | R-CACTCTGTCCCAGGACCAAT[i] |
| R-TTGGCCACTACTATGAGCAG | ||
| ZFX | Pecon-Slattery and O'Brien (1998) | |
| FCA018 | Menotti-Raymond et al. (1999) | |
| FCA240 | Menotti-Raymond et al. (1999) | |
| FCA311 | Menotti-Raymond et al. (1999) | |
| FCA478 | Menotti-Raymond et al. (1999) | |
| FCA651 | Menotti-Raymond et al. (1999) | |
| FCA674 | Menotti-Raymond et al. (1999) | |
| AMELY | same as AMELX (above) | F-CCCAGCACACTCCTATTTGG |
| R-GGAATTTCAGCTGCAAAGGA | ||
| DFFRY | same as DFFRX (above) | F-GCAGAATGCAGCAGACGTAA |
| R-CACCTGCAGGGGAAAGTGTA | ||
| DBY | same as DBX (above) | F-TGAAGAAACACTTCCAAACATTG |
| R-ACTGAAAATGTTGCTGGATGAA | ||
| SMCY | Murphy et al. (1999) | |
| SRY | Lyons et al. (1997) | |
| UBE1Y | Murphy et al. (1999) | F-ACCTTGGGGCAAATCATGTA |
| R-CCAAGCACTCCCTTTGTGAT | ||
| UTY | same as UTX (above) | F-CCTCTACACTTGCAGGAAGAA |
| R-GGGGAAAAGTTACTGACATGGA | ||
| ZFY | Pecon-Slattery and O'Brien (1998) | |
| Human | ||
| AMELX | Chen et al. (1999) | |
| DBX | GenBank accession no. G33988 | |
| DFFRX | RH database no. 47975 | |
| PLP | GenBank accession no. M54927 | |
| SMCX | F-CCACTCTCCTCCCCTCTTTT | |
| R-CTGAGGTGGTTGTGAGGACA | ||
| UTX | F-AACCAACTGGACGGAGAG | |
| R-TTCTGAAGGGGGAAAAGACA | ||
| ZFX | Chen et al. (1999) |
[i] Used in conjunction with first-round primer of opposite orientation.
[ii] Nomenclature used in Venta et al. (1996).
[iii] Abridged Universal Amplification Primer (GIBCO, BRL).