Comparison of TCA cycle, AAA lysine biosynthesis, and leucine biosynthesis related proteins. (A) Phylogenetic relationships among the amino acid sequence encoded in lys20 gene of T. thermophilus and high-scoring amino acid sequences from the result of homology search using PSI-BLAST (Altschul et al. 1997). A total of 332 amino acid sites were considered without gap regions in alignment. The bar indicates 10% difference of amino acid sequence. The DAD (DDBJ amino acid sequence database) accession nos. used in this phylogenetic analysis are D85684-1-1,D63999-62-1, Z46907-2-1, AE000772-5-1, U32779-6-1, AE000117-11-1,X71612-5-1, Z75208-75-1, U92974-14-1, U67561-1-1, AE000909-1-1,AE000922-3-1, U67500-4-1, AP000007-42-1, Z74230-1-1, Z74179-1-1,Z49114-1-1, AE000685-7-1, AE001091-11-1, U67579-2-1, AE000851-8-1, andAE001038-8-1. (B) Phylogenetic relationships among the amino acid sequence encoded in the hacA gene of T. thermophilus and the related sequences. A total of 279 amino acid sites were considered without gap regions in alignment. The DAD accession nos. used in this phylogenetic analysis are AP000007-41-1,AE000954-24-1, X99624-1-1, U46154-1-1, J05224-1-1, U87939-1-1,U80040-1-1, U17709-1-1, AE000756-5-1, AE000901-10-1, U67499-13-1,U67543-1-1, AE000967-2-1, AE000922-4-1, AE000714-13-1, U92974-16-1,Z99263-32-1, AL021287-2-1, AL031124-34-1, X84647-1-1, U32779-8-1,AE000117-9-1, Z99262-3-1, D67033-1-1, X53090-1-1, M33166-1-1, andD63833-1-1.