Table 2.

PCR Primer Sequences and Chromosomal Assignments of Chromosome 21p Markers

Locus Clone Type Primer sequences Size (bp) Chromosomal assignment
D21S190 pVC1.21cSTSCTGACAAGGAATTAGTAACC13313, 18, 21
TACACCTATTTGCCATTTGC
— ABM-C61STRCCTAGCTTTCTGTCAGCAGT45013, 14, 15,21, 22
CAACATGATTCCACATAATGTA
— hmc01a06ESTTGGATGTCACTCTCATCCTTG12213, 22,21, Y
TCGAAGAAGAACATCCATGAG
— ABM-C62DSTRCCACTGTTGTACAAATCAACTA14313, 15,21, Y
TCTAAGAATACCTGTAGGCATA
— Xba21STSCAATCCCGTTTCCCACG133N.D.
CTTGTTTGTGATGTGTGCCC
D21Z4 pTRA-4STSATTTGGAGGCCTTTGTGGCTATGG28713, 14, 21
GGCCTCAAAACGCTCCAAGTATCC
D21Z3 pTRA-1STSTGAGCCATTTGAGGCCTACTGTG13813, 14,21
TGTCTCCAAACTGCTCAATCAGAA
— ABM-C78STRCCTCTTCTATGCCAGTCTTAA1099, 13, 14, 15, 20, 21, 22
CAGCTCTAAAGTAAGCTTGGA
D21S5 pPW235DSTSCCAAAGGTTACTGCAGTCTC12313, 14,21
GGGGGTCATATAAAGGAAAC
D21S187 pVC10aSTSTACATGCAAATGACCAAGAG4004, 9, 13, 14, 15, 21, 22
ATCAGCAATGCCTTCTAAGT
D21S1276 pLSB77STSGGCCTGATGTCTGCCTTAGAT1081, 3, 9, 13, 14, 15, 20, 21, 22, Y
GCCATAGGTGAGCAACAGGA
D21S188 pVC1.12cSTRTTCTGTGTCATCTGAACTGG1849, 13, 14, 15, 20, 21, 22, Y
ACGCACATTGAATACTGAGG
D21S191 pVC1.23cSTSTGATTGGTATGACTTTGTTCCCAG1793, 4, 13, 14, 15, 21, 22
AGATGTCCACCCAGTGCTGC
D21S411 ABM-C74STRGAACCATTATAAGTTGACCATC196–2063, 4, 13, 14, 15, 21, 22
ACATGTTAACATGCTATATCTGT
D21S1277 pAW14aSTSAACTAAGGGCTCTAAAGCAT3003, 4, 9, 13, 14, 15, 21, 22, Y
AATGGGAGCACAAGTTCTAA
— 3C6STSCAAGAGGGCACTGAACAG591, 4, 13, 14, 15,21, 22, Y
GCACTCTTAGCTTTGCCA
D21S1278 pAW32aSTSTCATACAGAGTATAACACCAGGAC2661, 10, 13, 14, 15, 20, 21, 22, Y
GTCTTATTGTGATAGGCTTGC
rDNApCHB STSCGAGGTGACTCTCGGTTTGC37013, 14, 15, 21, 22
TTTCGCAAGCAGGCATTTG
rDNApA2 ESTTATGAACGCTTGGCCGC204N.D.
CGGTAACGCAGTGTCTAAGGC

[i] (N.D.) Not determined.