Table 1.

Genomic Distribution of the CAGGG Repeat

Library Name Coverage Positives No. of Est. Copy No. of Pstlbands
 2 cosmidLL02NC026.43354
10 cosmidLA10NC026.41021
15 cosmidLA15NCO16.563103
16 cosmidLA16NC025.993166
Human BACRPCI–1111.84994232
Human BACCIT-HSP3.41514432
Chimp BACRPCI-433.511132N.A.
Baboon BACRPCI-413.29618N.A.

[i] A summary of the number of CAGGG-repeat positive genomic clones identified after radioactive colony hybridization of 4 chromosome-specific cosmid libraries (human chromosomes 2, 10, 15 and 16) and four total-genomic BAC libraries (2 human, 1 chimpanzee and 1 baboon). Based on the depth of coverage of each library and the number of clones identified, an estimate of the haploid copy number was calculated for each chromosome and species. The number of distinctPstI restriction fragments hybridizing to a 900 bp CAGGG probe (p196.2.1) after Southern analysis of nylon-transferred panel of human monochromosomal somatic cell hybrid DNA is shown. The analysis generally confirms the non-uniform and multicopy distribution of the repeat element (note: the 900 bp CAGGG repeat fragment does not contain a PstI restriction site).