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Table 2.

Analysis of the Potential Sites for α2–Mcm1-Mediated Repression

ORF	Gene	bp	Sequence	mRNA[i]	Repression[ii]	Activation[iii]
mis = 0
YDR461W	MFA1	204	TGTAA TTACCCAAAAAGGAAATTTACA	a	100×	15×
YFL026W	STE2	201	TGTAAATTTCCTAATTGGGTAAGTACA	a	33×	28×
YGL032C	AGA2	271	TGTAATTTCCGAATACGGTAATTACA	a	167×	51×
YIL015W	BAR1	237	TGTAATTACCGTAAAAGGAAATTACA	a	81×	25×
YKL209C	STE6	184	TGTAATTACCTAATAGGGAAATTTACA	a	69×	17×
YNL145W	MFA2	223	TGTATTTACCTATTCGGGAAATTTACA	a	43×	47×
mis = 1
	DPS1		TGTAATTACTGTATTGGGAAATTTACA	a	404×	7.8×
YJL170C	ASG7	205	TGTAAATTTCCCGATGCGGTAATTACG	a	13×	14×
YJL169W	ORF	166		a, α, a/α
YKL188C	PXA2	259	TGTAATTAACGCCGAAGGAATATACA	a, α, a/α	1.3×	1.5×
YMR219W	ESC1	29	TGTAAATTCACCAAGGGGTTAAGTACA	a, α, a/α	1.1×	1.2×
YMR218C	ORF	191		a, α, a/α
mis = 2
	DPS2		TGTAATTACTTAAATAGGAAGTTTACA	a	283×	12×
YLR432W	ORF	405	TGTTCATTTCTAACTCTGGTTAATTACA	a, α, a/α	2.4×	0.9×
YGL187C	COX4	324	TGTATTTTTCAATAAGAGGATTATAACA	a, α, a/α	2.8×	1.0×
YNL053W	MSG5	249	TGTACTTTCCAAAAAAGGAAAATGTA	a, α, a/α	0.5×	22×
YGL075C	ORF	344	TGTCATATACCCGTGAAGGCATTTACA	a, α, a/α	1.3×	0.9×
YJL062W	ORF	162	TATAGATATGCTTATACGGATATATACA	a, α, a/α	2.3×	1.0×
YJR138W	ORF	645	TGTACAATACCATCAATGGCTTCTAAA	a, α, a/α	2.6×	1.4×

[i] mRNA was examined by Northern blot or RT–PCR in the previous studies and this work. (a) The transcript is only present in a cells. a, α, a/α) The RNA was present at the same level in haploid a, α, and diploida/α cells, respectively. The α2–Mcm1 sites that are involved in mating-type switching, DPS1 and DPS2,regulate cell-specific expression of several small transcripts (Szeto et al. 1997).

[ii] The fold repression is measured assaying the level of expression of a CYC1–lacZ reporter plasmid containing the designated α2/Mcm1-binding site and comparing it to the parent vector without the site (212 units) in a MATα strain.

[iii] The fold activation is measured assaying the level of expression of a CYC1–lacZ reporter plasmid with the designated α2/Mcm1-binding site lacking the CYC1 UAS sites and comparing it to the parent vector without the Mcm1 site (0.4 units) in a MAT a strain.