cDNAs, ESTs, and Primer Sequences Used in this Study
| Gene | EST (clone ID) | Size DNA fragment (bp) | Position of EST in the gene | Primers |
| PWP2 | EST 1 (627253) | 407 | 3′ UTR | 5′-TAAGTCTCATACAAAGTTCATGTG-3′ |
| 5′-GCTCCCTAGACCCGCTGGG-3′ | ||||
| KNP1 | EST 2 (365973) | 385 | 3′ UTR | 5′-TTCTGCCGTCTGAGGTTTTATA-3′ |
| 5′-GCACCAACGCTACCATCACT-3′ | ||||
| EST 3 (341284) | 363 | 3′ UTR | 5′-CTAACGCCAACACACTTTATTTC-3′ | |
| 5′-ACATTTATGGACCCATGAAAGGT-3′ | ||||
| AIRE | EST 4[i] | 549 | exon 14 | 5′-CTCGGTCTGGCCTGTGCCAT-3′ |
| 5′-CACAGGCAAGGAGAGGCTCCCGG-3′ | ||||
| EST 5[i] | 431 | exon 14 | 5′-CTCGGTCTGGCCTGTGCCAT-3′ | |
| 5′-ACTGACAAGAGGTGGCGCTGT-3′ | ||||
| cDNA (B1-1) | 1800 | |||
| C21orf3 | EST 6[ii] | 376 | 3′ UTR | 5′-AGCTGGATGCAGAGGGGCTG-3′ |
| 5′-GAGGACAGCCTGCAAAATCC-3′ | ||||
| EST 7[ii] | 956 | coding region | 5′-ATCTCCATTTGCCAGGAGATG-3′ | |
| 5′-GAGGACAGCCTGCAAAATCC-3′ | ||||
| cDNA (21919) | 2700 | |||
| SMT3A | EST 8 (340509) | 541 | 3′ UTR | 5′-AGGTTGGTAGGGAGGACAGAC-3′ |
| 5′-ACCCACGGGAGGAACATGAAG-3′ | ||||
| cDNA (L2328) | 1800 | |||
| C21orf1 | EST 9 (592718) | 461 | 3′ UTR | 5′-TCGCAGGTAGTAAACCGTTTTAT-3′ |
| 5′-ATAACATTCCTAGGCTGAAATTTC-3′ | ||||
| EST 10[iii] | 315 | 3′ UTR | 5′-GGACAGTCCTCTCGTCTTGC-3′ | |
| 5′-GACGCTGTCCGTGGTTTTAT-3′ |
[i] Selected from the AIRE cDNA.
[ii] Selected from C21orf3 cDNA.
[iii] Selected from C21orf1 cDNA.