Table 1.

Summary of FISH Data

Probe Positions covered[ii] Patient RB[iii] Patient MC[iii]
Initial screen
y133B06101–103++++
y934D03111–118+−++
y951C04120–124+−++
y918D08140–141+−+−
Secondary screens
y939H03106–109++N.D.
dJ213-J23108++N.D.
dJ104-A7109++N.D.
dJ101-N13++N.D.
dJ261-O4+−N.D.
dJ9-D18111+−N.D.
y975F11127–134N.D.++
dJ177-H4135N.D.+−

[i] Metaphase spreads from patients RB and MC were initially screened by FISH with a set of four YACs to give an approximate breakpoint location. This was followed by FISH experiments with YAC and PAC probes to give a higher resolution localization. YAC clones are prefixed by y; PAC clones by dJ.

[ii] The positions ordered in the YAC contig of Fig. 1 plus a location of each probe within it.

[iii] (++) Hybridization to both normal and deleted chromosomes; (+−) hybridization to normal chromosome only. (N.D.) Experiment not done.