RT–PCR analysis of ZNF208. RT–PCR analysis ofZNF208 from 12 human tissue sources confirmed the presence of two transcripts that result from the usage of alternative polyadenylation signals. The position of the primers and concomitant PCR product size are shown with respect to the ZNF208 gene structure. cDNA synthesis reactions without reverse transcriptase for each tissue served as a negative control (indicated by a minus sign).