Actual frameshift and total error rates for projects A and B. To calculate frameshift error rates, only insertions and deletions were counted. Mismatch errors, which account for the vast majority of errors after base 150, were included only in the total error count. Note that project B (▴,▵) has a slightly similar or slightly higher total error rate compared to project A (•,○) but only about one-third as many insertions and deletions up to base 500. For both projects, the frameshift error rate in the raw data is <1 in 1000 for >300 bases, and ≤1 in 10,000 for >100 bases in project B.