Schematic representation of the FMF transcript map. Positions of transcripts are shown relative to a composite genetic and physical map of the FMF region. Genetic markers are indicated by gray bars. All physical distances are indicated in kb. The map is drawn in three levels with resolution increasing on descending levels. Transcripts are not drawn to scale. (A) Transcripts identified by direct selection in the flanking intervals are indicated as are the physical map positions of large-insert genomic clones used for analysis. The position of cosmids subjected to partial DNA sequencing in the region telomeric of D16S468/D16S3070 and centromeric ofD16S3376 are also shown. (B) Transcripts identified using multiple methods are shown in the second level relative to a complete EcoRI restriction map of the interval. A contig of overlapping cosmids which were used for partial DNA sequence analysis are also shown, with those represented by blue lines sequenced at LANL and those in red sequenced at the NHGRI. Exon-trapping was performed on genomic DNA extending from cosmid 363D9 to PAC 273L24 (shown inA). (C) The 87-kb segment of genomic DNA sequenced to completion is indicated in purple. The organization of a tRNA cluster within this interval is indicated with the number, orientation, and map position of 18 tRNA genes. Nearby flanking Pol II transcripts are shown.