Markup | Genome Research

Table 1.

Computer-Assisted Value Selection for RED Optimization

Levels		Selection		Optimization 1–180		Optimization 1–360		Optimization 2 180/360
Levels		−	+	−α	0	+α	−α	0	+α	−α	−	0	+	+α
Ligation temp. (°C)	75	85	72	80	88	72	80	88	57	60	65	70	73
Ligation time (sec)	20	60		40			40		3	10	20	30	37
Number of cycles	200	800	50	500	950	50	500	950			500
Enzyme (units)	5	25		15		1	15	29	7	10	15	20	23
DNA (μg)	0.5	2.0	0.2	1.25	2.3		1.25				1.25
Oligonucleotide (ng)	50	150		100			100				100