Markup | Genome Research

Table 2.

The Results of SPMS Analysis of Steady-State PBGD mRNA Levels in AIP Patients

Mutation		Isolated total RNA[i]		Ratio of incorporated [³H]dNTP mutant allele/normal allele (±s.e.)[ii]		Mutant mRNA as a ratio of wild-type mRNA	Mean ±s.e. [iii]
Mutation		Isolated total RNA[i]		cDNA	genomic DNA	Mutant mRNA as a ratio of wild-type mRNA	Mean ±s.e. [iii]
	11/96	0.52 ± 0.007		0.97
517C → T	2/92	0.33 ± 0.030	0.55 ± 0.0067	0.61	0.41 ± 0.20
	2/92	0.12 ± 0.053		0.21
	2/97	0.34 ± 0.013		0.60
518G → A	2/97	0.36 ± 0.0081	0.57 ± 0.020	0.64	0.66 ± 0.038
	12/96	0.42 ± 0.044		0.73
	11/96	3.99 ± 0.089		0.82
673C → G	2/92	4.28 ± 0.064	4.85 ± 0.27	0.88	0.81 ± 0.075
	2/92	3.54 ± 0.076		0.73
	11/96	1.04 ± 0.041		0.24
673C → T	2/97	0.88 ± 0.025	4.34 ± 0.20	0.20	0.22 ± 0.012
	2/97	1.02 ± 0.11		0.23
	4/92	0.031 ± 0.010		<0.05
713T → G	4/92	0.012 ± 0.0021	0.72 ± 0.024	<0.05
	11/96	0.51 ± 0.0049		0.72
1073delA	5/94	0.12 ± 0.054	0.70 ± 0.017	0.17	0.27 ± 0.095
	5/94	0.25 ± 0.054		0.36

[i] Month and year of the isolation of total RNA.

[ii] Mean values of five estimates.

[iii] Mean value has been calculated from samples of the same duration of storage