Table 3.

List of ESTs Exhibiting Significant (P < 0.01) Differences in Abundance in the HL60 Cell Line Induced by DMSO or TPA

EST ID HL60 HL60 + TPA HL60 + DMSO Significance
418 22 10 1 3 × 10−7
211 24 10 2 4 × 10−7
198 23 2 8 × 10−7
356 16 2 0 3 × 10−6
380 12 1 0 6 × 10−5
1354 12 0 6 × 10−5
285 14 8 1 1 × 10−4
20150 11 0 2 × 10−4
244 0 1 14 3 × 10−4
293 13 6 1 3 × 10−4
292 11 0 15 × 10−4
650 14 5 2 5 × 10−4
335 15 3 3 9 × 10−4
444 10 4 1 2 × 10−3
1674 0 8 14 × 10−3
155 0 8 34 × 10−3
861 6 1 0 7 × 10−3
305 6 2 0 7 × 10−3
18060 6 0 7 × 10−3
18080 6 0 7 × 10−3
17660 6 0 7 × 10−3

[i] Only the probability (computed according to Equations { label needed for disp-formula[@id='E7'] } and { label needed for disp-formula[@id='E8'] }) corresponding to the most significant transition (numbers in bold) is listed (Okubo et al. 1995). The total EST numbers sampled from the HL60, HL60 + TPA and HL60 + DMSO cDNA libraries are 845, 845, and 1058, respectively. ESTs 418, 211, 356, 285, 293, 292, 650, 335, 444, 861, 305 corresponding to ribosomal proteins, and EST 380, a tag to an unkown gene, exhibit a marked reduction of expression level in the DMSO- and/or TPA-induced differentiated states. In constrast, ESTs 135 (ferritin), 2015 (LD78/macrophage inflammatory protein), 1674 (methionine adenosyltransferase), 155 (thymosin β-4), 1806 (lipocortin), 1808 (thymosin β-10), and 1766 (a metallothionein) appear more abundant in the TPA-induced state, also highly enriched in EST 19 (the ubiquitous elongation factor 1-α). β-Actin (EST 244), is the only markedly increased tag in the DMSO-induced state. EST numbers, abundance data, and protein assignments are from the “body map” public expression data repository athttp://www.imcb.osaka-u.ac.jp (K. Okubo and K. Matsubara).